Research Associate II

Job Summary

This position is responsible for developing hybridoma and antibody production in the New Clone Team (NCT) of Content Development. He/She will be involved in maintenance, expansion, examination, and storage of hybridoma clones and generation of monoclonal antibodies.

The duties include preparing culture and freezing related media and buffer, performing hybridoma and mammalian cell line cultures, purifying antibodies according to standard operating procedures, and performing Ab quality check by SDS and IEF.  Other job responsibilities include maintaining a clear notebook, log documents and records on FileMaker database, and performing calculations and manage the assigned function and tasks promptly with independence after appropriate training. 

In addition, this position also will communicate with the content culture team and screening team to ensure correct decision making by providing sufficient information.

Essential Functions

Reasonable accommodations may be made to enable individuals with disabilities to perform the essential functions.

• Under guidance of senior personnel, prepare hybridoma culture-related material and antibody purification-related buffer
• Hybridoma cell culture maintenance and expansion
• Characterize antibody level in hybridoma culture supernatant by Octet in accordance with SOP
• Characterize monoclonal clonal antibodies isotype and mycoplasma contamination by ELISA and commercial kit/PCR, respectively
• Characterize monoclonal clonal antibodies purity by SDS and IEF electroporation system
• Analyze and summarize data with high attention to detail in accordance with SOPs
• Complete all documentation, including project conceptual design, batch records, experimental data, Clarity and FileMaker
• Lab database organization and maintenance
• Collaborate with other product development Research Associates and Scientists to provide project updates, to establish project timelines and to assist completion of product development documentation
• Other projects or responsibilities as assigned

iACT Competencies

• innovate – Be better, keep improving, be more efficient, creative, and be in the forefront of development
• Aspire – Have desire, purpose, and ambition challenging ourselves to push the limits and reach new heights
• Collaborate – Foster teamwork, common goals, selflessness, communication and mutual support
• Transform – Adapt, learn, re-invent and change for future development and growth

Minimum Qualifications – Education and Experience

• Bachelor of Science or equivalent degree with a course works in cell biology or relate field.

Preferred Qualifications – Education and Experience

• Mammalian cell culture and cell line maintenance;
• Basic knowledge of immunology including but not limited to antibody and antigen interaction is a plus;
• Basic knowledge of protein purification is a plus.

Work Environment & Physical Demands

Lab

While performing the duties of this job, the employee regularly works in a laboratory environment with occasional data entry on a computer. Occasional exposure to a warehouse environment.

The physical demands described here are representative of those that must be met by an employee to successfully perform the essential functions of this job.

While performing the duties of this job, the employee is regularly required to use hands and fingers to handle, feel, or operate objects, tools or controls, and reach with hands and arms. The employee is frequently required to stand, talk and hear.